Sharp PC-9300 Technical Information

Modulus™ Microplate Multimode Reader Operating Manual Part Number 998-9300 Revision 1.6

1 Instrument www.turnerbiosystems.com 5 NOTE: Do not connect anything except the injector system to the

1 Instrument www.turnerbiosystems.com 6 1.2 Touch Screen Basics A built-in Windows-based PC with a custom interface enables user-frie

1 Instrument www.turnerbiosystems.com 7 • Visible when a USB flash drive is detected • Icon turns dark when files have been saved to

1 Instrument www.turnerbiosystems.com 8 • When viewing Absorbance results, the button toggles between the different data formats: OD,

1 Instrument www.turnerbiosystems.com 9 maximum volume per well is 250 µl. Overfilling a well will result in spills and possible damage

1 Instrument www.turnerbiosystems.com 10 1.3 HOME screen The HOME screen contains four options: • NEW PROTOCOL launches the wizard t

1 Instrument www.turnerbiosystems.com 11 • NEW PROTOCOL allows the user to create new protocols. Touching this button launches the NEW

1 Instrument www.turnerbiosystems.com 12 8. To save the modified protocol: 1. Touch the white text button with the protocol name. See

1 Instrument www.turnerbiosystems.com 13 1.5.2 Well Selection To select or deselect a well, touch the well to toggle between green a

1 Instrument www.turnerbiosystems.com 14 Opening the instrument door while the Microplate Sample Tray is in motion will cause it to st

Turner BioSystems, Inc. and its suppliers own the written and other materials in the Modulus Microplate Reader operating manual

1 Instrument www.turnerbiosystems.com 15 Use the DELETE button to permanently remove unwanted results from the re

1 Instrument www.turnerbiosystems.com 16 1.7 Transferring Data Results files are transferred to a PC or Mac via a USB flash drive usi

1 Instrument www.turnerbiosystems.com 17 1.8 Repeat Runs Each detection modality has the option to do repeat runs. Injectors cannot b

2 Luminescence www.turnerbiosystems.com 18 2 Luminescence 2.1 General Information • The Luminometer detection wavelength range is 350 to 65

2 Luminescence www.turnerbiosystems.com 19 2.4 Starting a Run Easy-to-use protocol wizards can be accessed from the HOME screen to set up the

2 Luminescence www.turnerbiosystems.com 20 2.5 Kinetic In kinetic mode, the instrument performs multiple reads of a single well with

2 Luminescence www.turnerbiosystems.com 21 2.7 Using a Standard Light Plate The optional Standard Light Plate provides a quick way to

2 Luminescence www.turnerbiosystems.com 22 2.8 Luminescence Data Luminescence data is displayed in Relative Luminescence Units (RLU).

www.turnerbiosystems.com 23 3 Fluorescence 3.1 General Information • Microplate Recommendations: - Black plates are highly recommended. -

3 Fluorescence www.turnerbiosystems.com 24 3.3 Starting a Run Easy-to-use protocol wizards can be accessed from the HOME screen to set

Table of Contents 1 Instrument ...

3 Fluorescence www.turnerbiosystems.com 25 3.4 Switching Fluorescence Optical Kits 1. Close the instrument door by touching the DOOR

3 Fluorescence www.turnerbiosystems.com 26 5. Hand tighten the 2 captive screws using the Allen Wrench (size 7/16) provided with the ki

www.turnerbiosystems.com 27 4 Absorbance 4.1 General Information • Four installed filters and two customizable filter holders are included on

4 Absorbance www.turnerbiosystems.com 28 4.2.1 Customizable Absorbance Paddle Two absorbance paddles are included for customization. T

4 Absorbance www.turnerbiosystems.com 29 3. Touch the white text button to select a protocol or modify the displayed parameters. See Fi

4 Absorbance www.turnerbiosystems.com 30 Wells marked with a Ref were designated as Reference wells. Reference value of each wavelength

4 Absorbance www.turnerbiosystems.com 31 9. To ensure the instrument detects the newly installed module, verify the ABSOR icon appears

www.turnerbiosystems.com 32 5 Injector System 5.1 Injector System Components Unpack the Modulus™ Microplate Reader injector system carefully. V

5 Injector System www.turnerbiosystems.com 33 1. Power off the Modulus™ Microplate Reader. 2. Place the Inject

5 Injector System www.turnerbiosystems.com 34 6. Manually open the instrument door and hold it down. Insert the injector tip into the

Table of Contents 2.9 Luminescence Module ...

5 Injector System www.turnerbiosystems.com 35 11. Pinch the lever on the back of the inlet tube holder to clip the stainless steel tube

5 Injector System www.turnerbiosystems.com 36 NOTE: The reagent container holder can accommodate four size containers: A round flat bott

5 Injector System www.turnerbiosystems.com 37 5.3.1 Priming Injectors 1. Place the reagent container into the reagent holder. 2.

5 Injector System www.turnerbiosystems.com 38 2. Place the container of 70% ethanol on the bottle holder. The stainless steel inlet tub

5 Injector System www.turnerbiosystems.com 39 5.8 Removing or Replacing Inlet and Outlet Plastic Tubing Replacement tubing assembly is availab

www.turnerbiosystems.com 40 6 Accessories 6.1 Curve Fitting Data Analysis Software (9300-970) Curve Fitting Data Analysis software is easy-to-us

6 Accessories www.turnerbiosystems.com 41 • When an external PC has control of the instrument, the Modulus™ Microplate Reader touch s

6 Accessories www.turnerbiosystems.com 42 3. Power on the Modulus™ Microplate Reader. 4. The FOUND A NEW HARDWARE wizard will appe

6 Accessories www.turnerbiosystems.com 43 6.2.7 Connecting to a PC Using an RS-232 Serial Cable 1. Power on the instrument and PC.

6 Accessories www.turnerbiosystems.com 44 6.3.3 Changing the Battery 1. Remove the two Phillips head screws. 2. Remove the batter

Table of Contents 6 Accessories ...

www.turnerbiosystems.com 45 7 Tools From the INSTRUMENT CONTROL screen, touch the TOOLS button to manage various aspects of the instrument. 7.1

7 Tools www.turnerbiosystems.com 46 7.2 Event Log The event log can be downloaded onto a USB flash drive in a .TXT format. Insert

7 Tools www.turnerbiosystems.com 47 7.6 Updating Firmware Perform a firmware update only when instructed by Turner BioSystems Tech

www.turnerbiosystems.com 48 8 Instrument Maintenance 8.1 General Instrument Care The instrument must be on a level surface to operate properly.

8 Instrument Maintenance www.turnerbiosystems.com 49 8.3 Touch Screen Care Do not use any sharp object such as a pen, pencil, sty

www.turnerbiosystems.com 50 9 Troubleshooting 9.1 Table of Error Messages Error Message Description Resolution Start Problem Instrument busy T

9 Troubleshooting www.turnerbiosystems.com 51 Injector 2 not installed The protocol requires addition of reagent using Injector 2 but it is not d

9 Troubleshooting www.turnerbiosystems.com 52 9.2 Table of Common Problems Symptom Possible Cause Resolution Injections sputter, dr

9 Troubleshooting www.turnerbiosystems.com 53 9.3 Commonly Asked Questions 9.3.1 PC Connectivity Q: When I connect the Modulus™ M

www.turnerbiosystems.com 54 APPENDIX Appendix A - Specifications General Specifications • Detection Modes: Luminescence, Fluorescence, and Abs

Table of Contents APPENDIX ...

APPENDIX www.turnerbiosystems.com 55 Injector System Specifications • Number of Injectors: single or dual injectors • Dispense Volume

APPENDIX www.turnerbiosystems.com 56 Appendix B - Warranty and Obtaining Service General Warranty Information Turner BioSystems warra

APPENDIX www.turnerbiosystems.com 57 Prior correspondence is necessary to: • Eliminate minor problems, easily handled on-site in your

APPENDIX www.turnerbiosystems.com 58 Appendix C - Ordering Information To get a price quote or place an order call the Turner BioSyst

APPENDIX www.turnerbiosystems.com 59 Part Number Description Includes Replacement Part 159-9100 Power supply Brick 24V, 60W, 2.5A 046-

APPENDIX www.turnerbiosystems.com 60 Appendix D - How to Contact Us Turner BioSystems, Inc. 645 North Mary Avenue Sunnyvale, CA 94085

www.turnerbiosystems.com 61

645 N. Mary Avenue • Sunnyvale, CA 94085 Phone: (408) 636-2400 •Toll Free: (888) 636.2401 • Fax: (408) 737-7919 www.turnerbiosystems.com

www.turnerbiosystems.com 2 1 Instrument 1.1 Getting Started 1.1.1 Description Thank you for purchasing the Turner BioSystems, Inc. Modulus™ Mic

www.turnerbiosystems.com 3 1.1.3 Unpacking and Inspection Upon receiving the Modulus™ Microplate Reader, inspect it carefully for any damage to the

1 Instrument www.turnerbiosystems.com 4 3. Plug the power line cord into the power connector on the back panel of the instrument. Plug